We use IFS data of four galaxies from the TIMER survey to explore the kinematics measured in different spectral regions that are sensitive to distinct stellar populations. We derive the velocity and velocity dispersion of both a young and an old stellar population and several stellar parameters. We report a correlation of the mean luminosity-weighted age with the difference in the kinematical parameters of the blue and red ranges of the spectrum, which are dominated by young and old stellar populations, respectively. Young stellar populations, located primarily in nuclear rings, exhibit in general higher values of velocity and velocity dispersion than the old stellar population.We also report trends with age and extinction. Our study demonstrates that kinematic differences caused by different stellar populations can be identified in the central regions of nearby galaxies even from intermediate resolution spectroscopy.