There is increasing evidence that single-star evolutionary models are unable to reproduce all of the observational properties of massive stars. Binary interaction has emerged as a key factor in the evolution of a significant fraction of massive stars. In this study, we investigate the helium (Y(He)) and nitrogen surface abundances in a comprehensive sample of 180 Galactic O-type stars with projected rotational velocities of ≤150 km/s. We found a subsample (~20% of the total, and ~80% of the stars with Y(He) ≥ 0.12) with a Y(He) and nitrogen abundance combined pattern that is unexplainable by
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